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Xoα●????鋓ワ「U@?ゥ6bGネツW]2ノセイF&ル・&K@ョd テ部ワO摩,ノJ@2ノ.?0Yイp% ョ、艤イd HV2蝕vi艨ノ??+ネp%%% K@イ吹LイK#L摩,\ ネ@?)ケ?Y瀦? d綻y`イd 濳?翻?ェ/8yIENDョB`? ソAタ@@   wjCBID Primer NamePrimer SequenceNoteCB000135sfFor people work with plantCB00023'AOX17For Pichia vectors with AOX1 terminator, reverse primerCB00035'AOX15For Pichia vectors with AOX1 promoter, forward primerCB0004AC5,Drosophila Actin 5C promoter, forward primerCB0005 Alpha-factor,Alpha factor signal sequence, forward primerCB0006Amp-R45' end of ampicillin resistance gene, reverse primerCB0007 AUG1-Forward5For Pichia vectors with AUG1 promoter, forward primerCB0008 AUG1-Reverse5For Pichia vectors with AUG1 promoter, reverse primer0Bovine growth hormone terminator, reverse primerCB0010Bglob-intron-F)Rabbit beta-globin intron, forward primerCB0011 Bglob-pA-R/Rabbit beta-globin polyA region, reverse primerCB0012CAT-R95' end of chloramphenicol resistance gene, reverse primerCB0013 CMV Forward2Human CMV immediate early promoter, forward primerCB0014CRE-R)5' end of Cre recombinase, reverse primerCB0015CYC15CYC1 transcription termination signal, reverse primerCB0016DsRed1-C 3' end of DsRed1, forward primerCB0017DsRed1-N 5' end of DsRed1, reverse primerCB0018 EBV Reverse%SV40 polyA terminator, reverse primerCB0019Ecdysone Forward.Drosophila heat shock promoter, forward primerCB0020 EF-1a Forward3Human elongation factor-1a promoter, forward primerCB0021EGFP-C3' end of EGFP, forward primerCB0022EGFP-N5' end of EGFP, reverse primerCB0023EXFP-R7For distinguishing EGFP vs ECFP vs EYFP, reverse primerCB0024GAL1+S. cerevisiae GAL1 promoter, forward primerCB0025 Gal10pro-F,S. cerevisiae GAL10 promoter, forward primerCB0026 Gal4-N-term13' end of Gal4 DNA binding domain, forward primerCB0027Gal4-AD03' end of Gal4 activation domain, forward primerCB0028GFP-F3' end of GFP, forward primerCB0029GFP-R5' end of GFP, reverse primerCB0030GPDpro-F*S. cerevisiae GPD promoter, forward primerCB0031GW-3'*3' end of Gateway cassette, forward primerCB0032GW-5'*5' end of Gateway cassette, reverse primerCB0033H1!Human H1 promoter, forward primerCB0034HA-FHA tag, forward primerCB0035HA-RHA tag, reverse primerCB0036HAT&Histidine affinity tag, forward primerCB0037hGH-PA-R/Human growth hormone terminator, reverse primerCB0038IRES-F3' end of IRES, forward primerCB0039IRES-R5' end of IRES, reverse primerCB0040L4440)5' of MCS in L4440 vector, forward primerCB0041LacZ-R5' end of LacZ, reverse primerCB0042LexA13' end of LexA DNA binding domain, forward primerCB0043LKO.1 5'!Human U6 promoter, forward primerCB0044LNCX"Human CMV promoter, forward primerCB0045Luc-F$3' end of luciferase, forward primerCB0046LucNrev$5' end of luciferase, reverse primerCB0047M13 (-21) Forward In lacZ geneCB0048 M13 (-40)CB0049 M13 ReverseCB0050M13/pUC ForwardCB0051M13/pUC ReverseCB0052MBP-F13' end of maltose binding protein, forward primerCB0053 MT Forward3Drosophila metallothionein promoter, forward primerMMLV-F:Moloney murine leukemia virus LTR (MoMuLV), forward primerCB0055mPGK-F"Mouse PGK promoter, forward primerCB0056MSCV&Murine stem cell virus, forward primerCB0057MSCV-rev&Murine stem cell virus, reverse primerCB0058MT1-F0Mouse metallothionein 1 promoter, forward primerCB0059mU6-F!Mouse U6 promoter, forward primerCB0060MycMyc tag, forward primerCB0061Neo-F23' end of neomycin resistance gene, forward primerCB0062Neo-R25' end of neomycin resistance gene, reverse primerCB0063NOS-F*Nopaline synthase promoter, forward primerCB0064 OpIE2 ForwardOpIE2 promoter, forward primerCB0065pACYC-Fp15A origin, forward primerCB0066pAd-CMV.For cloning sites after SalI in pAd-CMV vectorCB0067pBABE 3'9SV40 enhancer, 3' of MCS in pBABE vectors, reverse primerCB0068pBABE 5'@Psi packaging signal, 5' of MCS in pBABE vectors, forward primerCB0069 pBAD Forward8For vectors with E. coli araBAD promoter, forward primerCB0070 pBAD Reverse8For vectors with E. coli araBAD promoter, reverse primerCB0071 pBluescriptKSFor pBluescript vectorCB0072 pBluescriptSKCB0073pBMN 5'4MMLV sequence, for inserts in pBMN retroviral vectorCB0074 pBRforBam7In pBR322 tet region, upstream of BamHI, forward primerCB0075 pBRforEco0In pBR322, upsteam of EcoRI site, forward primerCB0076 pBRrevBam9In pBR322 tet region, downstream of BamHI, reverse primerCB0077pCAG-F<Rabbit beta-globin intron, for pCAG plasmids, forward primerCB0078 pCasper-F45' end of Drosophila mini-white gene, reverse primerCB0079pcDL-F/5' of EcoRI site in pcDL vector, forward primerCB0080pENTR-F+5' of attL1 in pENTR vector, forward primerCB0081pGEX 3')3' of MCS in pGEX vectors, reverse primerCB0082pGEX 5'33' end of glutathione-S-transferase, forward primerCB0083pGP704-R<R6K gamma origin, 3' of MCS in pGP704 vector, reverse primerCB0084pHybLex ReverseADH terminator, reverse primerpLTet-F9Lambda phage early leftward (pL) promoter, forward primerCB0086pLXSN 5'4Murine stem cell virus, same as MSCV, forward primerCB0087 pMRB101-F7HCMV major immediate-early protein (IE), forward primerCB0088pMT2-F*3' end of synthetic intron, forward primerCB0089 pMX-S18117MMLV sequence, 5' of MCS in pMXs vector, forward primerCB0091Polyhedrin reverse?For baculovirus vector with polyhedrin promoter, reverse primerCB0092 pQE promoter(5' of MCS in pQE vectors, forward primerCB0093 pREP Forward1Rous sarcoma virus (RSV) promoter, forward primerCB0094 pRS-marker2To sequence yeast selectable marker in pRS vectorsCB0095Pry1PZ P-element, reverse primerCB0096pTrcHis Forward+5' of MCS in pTrcHis vector, forward primerCB0097pTrcHis Reverse;3' of MCS in pTrcHis vector, same as pBAD-R, reverse primerCB0098Puro-F33' end of puromycin resistance gene, forward primerCB0099pZIP,Murine leukemia virus (MuLV), reverse primerCB0100RCAS-F73' of Rous sarcoma virus (RSV) env gene, forward primerCB0101 RVprimer3(5' of MCS in pGL3 vector, forward primerCB0102SFFV-F1Spleen focus forming virus 5' LTR, forward primerCB0103SP6SP6 promoter, forward primerCB0104SV40pA-RSV40 polyA, reverse primerCB0105 SV40pro-F$SV40 promoter/origin, forward primerCB0106 SV40-spliceRCB0107T3T3 promoter, forward primerCB0108T7T7 promoter, forward primerCB0109 T7 TerminalT7 terminator, reverse primerCB0110 Tac promoterTac promoter, forward primerCB0111TRC-FCB0112Ubx-F-Drosophila Ultrabithorax gene, forward primerCB0113 V5 ReverseV5 epitope, reverse primerCB0114WPRE-R5' end of WPRE, reverse primerCB0115Xpress ForwardXpress epitope, forward primerCB0116 GLprimer1for pGL vectorCB0117 GLprimer2CB0118CB0119 RVprimer4CB0120Luc_U111"reverse primer for Luciferase geneCB0121CmF2"Chlorphenicol gene, forward primerCB0122Neo_D545Neomycin forward primerCB0123RepO-Fhouse keeping, pBR322 originCB0124RepO-RCB0125CB0126 pRLuc-MCS-RCB0127pSicoFCB0128pSicoRCB0129MBP-RCB0130FLAPCB0131MalECB0132Met25CB0133DsRed1-FCB0134pFastBacForwardCB0135pFastBacReverseATGACGCACAATCCCACTATC 7GCAAATGGCATTCTGACATCC  7GACTGGTCCAATTGACAAGC  7ACACAAAGCCGCTCCATCAG  7TACTATTGCCAGCATTGCTGC  7ATAATACCGCGCCACATAGC 7CAATTTACATCTTTATTTATTAACG  7GAAGAGAAAAACATTAGTTGGC  7TAGAAGGCACAGTCGAGG  7CTGGTCATCATCCTGCCTTT 7TTTTGGCAGAGGGAAAAAGA 7GCAACTGACTGAAATGCCTC 7CGCAAATGGGCGGTAGGCGTG  < 7GCAAACGGACAGAAGCATTT 7GCGTGAATGTAAGCGTGAC  7AGCTGGACATCACCTCCCACAACG  7GTACTGGAACTGGGGGGACAG  7GTGGTTTGTCCAAACTCATC  7CTCTGAATACTTTCAACAAGTTAC  7TCAAGCCTCAGACAGTGGTTC  7CATGGTCCTGCTGGAGTTCGTG  7CGTCGCCGTCCAGCTCGACCAG  7GTCTTGTAGTTGCCGTCGTC  7AATATACCTCTATACTTTAACGTC  7GGTGGTAATGCCATGTAATATG  7GAGTAGTAACAAAGGTCAA 7AATACCACTACAATGGAT 7GGTCCTTCTTGAGTTTGTAAC 7CCATCTAATTCAACAAGAATTGGGACAAC  7CGGTAGGTATTGATTGTAATTCTG 7GCATGATGACCACCGATATG 7AATCTCGCCGGATCCTAACT 7TCGCTATGTGTTCTGGGAAA 7TACCCATACGACGTCCCAGA 7TCTGGGACGTCGTATGGGTA 7GAGGAGCACGCTCATGCCCAC  7TGGCTCTCCTCAAGCGTATT 7CCTCACATTGCCAAAAGACG 7AGCGAGTCAGTGAGCGAG  7GACAGTATCGGCCTCAGGAA 7CGTCAGCAGAGCTTCACCATTG  7GACTATCATATGCTTACCGT 7AGCTCGTTTAGTGAACCGTCAGATC  7AGTCAAGTAACAACCGCGA 7TGTAAAACGACGGCCAGT 7GTTTTCCCAGTCACGAC 7CAGGAAACAGCTATGAC 7CCCAGTCACGACGTTGTAAAACG  7AGCGGATAACAATTTCACACAGG 7GATGAAGCCCTGAAAGACGCGCAG  7CATCTCAGTGCAACTAAA  7ATCAGTTCGCTTCTCGCTTC 7CATTCTGCACGCTTCAAAAG 7CCCTTGAACCTCCTCGTTCGACC  7CAGCGGGGCTGCTAAAGCGCATGC 7GCTGTCCTCTAAGCGTCACC 7CAGCACAAAAGGAAACTCACC 7GCATCAATGCAGAAGCTGATCTCA 7CGTTGGCTACCCGTGATATT 7GCCCAGTCATAGCCGAATAG 7GCGTTCAAAAGTCGCCTAAG 7CGCAACGATCTGGTAAACAC  7TGAAGTCAGCCCCATACGAT 7GCTAGAGATCTGGTACCGTC 7ACCCTAACTGACACACATTCC  7CTTTATCCAGCCCTCAC  7ATGCCATAGCATTTTTATCC  7GATTTAATCTGTATCAGG  7TCGAGGTCGACGGTATC 7TCTAGAACTAGTGGATC 7GCTTGGATACACGCCGC 7CTTGGAGCCACTATCGAC 7AATAGGCGTATCACGAGGC 7GGTGATGTCGGCGATATAGG 7GCAACGTGCTGGTTATTGTG 7GGGTTTTATTAACTTACAT  7GTTGCCTTTACTTCTAGGCCT  7CTACAAACTCTTCCTGTTAGTTAG 7CCGGGAGCTGCATGTGTCAGAGG 7GGGCTGGCAAGCCACGTTTGGTG 7AACAAGCCAGGGATGTAACG 7GAGTCACTTTAAAATTTGTATACAC  7ACTGAGCACATCAGCAGGAC 7AAGATGCAGGCAGCTGAGTT 7TTGCCTTTCTCTCCACAGGT 7GACGGCATCGCAGCTTGGATACAC  7AAATGATAACCATCTCGC  7GTCCAAGTTTCCCTG  7CCCGAAAAGTGCCACCTG  7GCTCGATACAATAAACGCC  7CGGCATCAGAGCAGATTGTA 7CTTAGCATGTCCGTGGGGTTTGAAT 7GAGGTATATATTAATGTATCG  7GCAACCTCCCTTCTACGAGC 7TCCTTTCCAGCGAGGTTCTA 7ACATGGGTGGTGGTATAGCGCTTGCG 7CTAGCAAAATAGGCTGTCCC  7ATTGATTGACTGCCCACCTC 7GATTTAGGTGACACTATAG 7GAAATTTGTGATGCTATTGC 7TATTTATGCAGAGGCCGAGG 7CACAAAGATCCGGACCAAAG 7GCAATTAACCCTCACTAAAGG 7TAATACGACTCACTATAGGG 7GCTAGTTATTGCTCAGCGG 7GAGCGGATAACAATTTCACACAGG  7CAAGGCTGTTAGAGAGATAATTGGA  7AACTCGTACTTTGAACAGGC 7ACCGAGGAGAGGGTTAGGGAT  7CATAGCGTAAAAGGAGCAACA 7TATGGCTAGCATGACTGGT 7TGTATCTTATGGTACTGTAACTG 7CTTTATGTTTTTGGCGTCTTCCA 7CTAGCAAAATAGGCTGTCCC 7GACGATAGTCATGCCCCGCG 7AGGAACCAGGGCGTATCTCT 7TCCGGCCTTTATTCACATTC 7GCTTCCTCGTGCTTTACGGT 7GTGTAGGTCGTTCGCTCCA 7TCCTGTTACCAGTGGCTGCT 7GGGCGGTAGGCGTGTACGGTGG 7CGTAGTAGTTGATGAAGCTGTCC 7GAGGCTTAATGTGCGATAAAAGA 7TTATGTAACGCGGAACTCCA 7GGTCGGCAGTACCGTTACAC 7CAGTGCAGGGGAAAGAATAGTAGAC 7GGTCGTCAGACTGTCGATGAAGCC 7CTTCGTGTAATACAGGGT 7AGGACGGCTGCTTCATCTAC 7GGATTATTCATACCGTCCCA 7CAAATGTGGTATGGCTGATT 7CB0136CB0137CB0138CB0139CB0140 PBV220+ 7 PBV220- 727FAGA?GTT?TGA?TCM?TGG?CTC?AG 71492R 7TACGGYTACCTTGTTACGACTT 7 XW-F1 7XW-F2XW-F3CCACACATTATACGAGCCGGAAG 7CAAAGATCGAGACCGAGACG 7AAATACCCATCGCTGCAACG 7 GGGCAGCATTCAAAGCAG 7 TTATCAGACCGCTTCTGC 7CB0141CB0142CB0143CB0144CB0009 7 BGH-Reverse 7CB0145 M13(-47)FCGCCAGGGTTTTCCCAGTCACGACCB0147CB01480  T揚w顛uirムy?b gP僕QKm戎_irRh  /  7\ Comate Biosciences Co. DNA Sequencing Primer List 7CB0150CB0151tRNA-U6pCold-FCGCCATATCGCCGAAAGGpCold-RGGCAGGGATCTTAGATTCTGCB0152CB01535'BD\GAL4-BD-Cfor$SV40 splice sequence, reverse primer 7 pRLuc-MCS-F 7CCAGCTTGGTTCCCAATAGA 7CB0090 7Polyhedrin forward 7#Polyhedrin promoter, forward primer 7CB0054 7CCTTATGCAGTTGCTCTCC 7CB0085 7CB0155CB0156CB0157CB0158CB0159CB0160CB0161CB0162CB0163CB0164CB0165CB0166GCCCTCATAGTTAGCGTAACGCB0167AGATGGTGCACGATGCACAGpCAGGS-F 7TTCGGCTTCTGGCGTGTGAC 7pCAGGS-R 7TATGTCCTTCCGAGTGAGAG 7pLNCX-F 7pLNCX-R 7ACCTACAGGTGGGGTCTTTCATTCCC 7XW-R1 7lCATTATCGCACGGAATGCCAAG 7lLX-His 7lGAACCATTGCAACTTGGCCT 7lCB0146 7lRV-M 7lGAGCGGATAACAATTTCACACAGG  7lS-Tag(69945-3) 7lGAACGCCAGCACATGGACA 7l NEW-BGH-r 7lCAgggTCAAggAAggCAC 7lCB0149 7l315F 7lacgtgggtataagaggcg 7l315R 7lcgatgctagacgatccag 7ltacgatacaaggctgttagagag 7lCB0154 7l3`AD 7lM13(-96) 7lTCATCGGAAGAGAGTAG 7l pGAL4-AD-rev 7lGAACTTGCGGGGTTTTTC 7l pGAL4-BD-rev 7lAATCATAAGAAATTCGCCC 7lCMV-R 7lGTTCACGGTGCCCTCC 7lU6 7lCAGTGCAGGGGAAAGAATAGTAGA 7lC-CMV-24 7lTATTAGGACAAGGCTGGTGGGCAC 7lN-CMV-30 7lAATGTCG<ニTAATAACCCCGCCCCGTTGACGC 7l U6-promoter?human ?  wlCCGTAACTTGAAAGTATTTCG wlAGCTCGTTTAGTGAACCGTCAGATCG 7?r 「 ┐pO」;」・fヲR ァクァ、aィM&ゥョゥVェB讙メ[ォG ? ァャ %ュ サュァ ョ  弍 ー マーサoア[イ ウ楜}エi,オ邨モカカ「鵡Bク.ケヨケツ?sKサ7扈モgシS箴ホiスU?スVセB鮴ユLソ8汰}眩ヘチkツ;リティEナ淅oノ]ノ-ソハフ iヘ9 メホ「 +ミ 禾Y?ソLヤーユ?、ヨt~ラNヲリvル[レ+?フ-ワ?uワEダ:゚ S#j:チmcc||kSホ?A} 00_)ef[$ャ -}A} 00_)ef[$ャ -}A} 00_)ef[$ャ -}A} 00_)ef[$ャ -}A} 00_)ef[$ャ -}A} 00_)ef [$ャ -}A} 00_)フL[$ャ -}A} 00_)フL[$ャ -}A} 00_)フL[$ャ -}A} 00_)フL[$ャ -}A} 00_)フL[$ャ -}A} 00_)フL [$ャ -}A} 00_)23[$ャ -}A} 00_)23[$ャ -}A} 00_)23[$ャ -}A} 00_)23[$ャ -}A}  00_)23[$ャ -}A}! 00_)23 [$ャ -}-}# 00_)}A}$ 00_)[$ャ -}A}% 00_)??[$ャ -}A}& 00_)23[$ャ -}-}' 00_)}A}( ?00_)?ヌホ?[$ャ -}(})  00_)}A}* a?00_)ニ??[$ャ -}U}+ 00_)[$ャ -##0.}}. 僘?00_)?[$ャ -?##0.?-"?? ?_ ?}}/ 00_)・・・?[$ャ -????##0.????-"?? ????_ ????}-}0 ?00_)}-}1 ??00_)}A}2 僘?00_)???[$ャ -}A}5 00_)[$ャ -}A}6 00_)[$ャ -}A}7 00_)[$ャ -}A}8 00_)[$ャ -}A}9 00_)[$ャ -}A}: 00_) [$ャ -}A}; 彳?00_)???[$ャ -}}< ????00_)?[$ャ -????##0.????-"?? ????_ ????}}= ??v?00_)?フ?[$ャ -?##0.?-"?? ?_ ?}(}>  00_)}x}???フ?00_)イイイ??フ?[$イイイ??## イイイ??-" イイイ??_ }(}B ?00_)}(}M ?00_)}(}O ?00_)}(}Q????00_)}<}R ?00_)?????フ?[$}(}U???00_)}(}V???00_)}(}W???00_)}d}X?00_)??フ?[$ ??## ??-"}(}Z00_)}(}[00_)}(}\00_)}(}]?タ?00_)}(}^?タ?00_)}(}_?タ?00_)}(}`???00_)}(}a???00_)}(}b00_)}x}c00_)??フ?[$??## ??-" ??_ }(}d漬P?00_)}(}e漬P?00_)}x}f漬P?00_)??フ?[$??## ??-" ??_ }(}g漬P?00_)O?20% - :_欠eW[恫r 1 efロ褥? ?%O"?20% - :_欠eW[恫r 2 efワ? ?%O&?20% - :_欠eW[恫r 3 ef?ン? ?%O*?20% - :_欠eW[恫r 4 ef裄? ?%O.?20% - :_欠eW[恫r 5 efロⅴ? ?%O2?20% - :_欠eW[恫r 6  ef?鰮? ?%O?40% - :_欠eW[恫r 1 フLクフ? ?%O#?40% - :_欠eW[恫r 2 フL貉ク? ?%O'?40% - :_欠eW[恫r 3 フLラ莨? ?%O+?40% - :_欠eW[恫r 4 フLフタレ? ?%O/?40% - :_欠eW[恫r 5 フLカン? ?%O3?40% - :_欠eW[恫r 6  フL?エ? ?%O ?60% - :_欠eW[恫r 1 23糞ラ? ????%O$?60% - :_欠eW[恫r 2 23ル藍? ????%O(?60% - :_欠eW[恫r 3 23ツヨ? ????%O,?60% - :_欠eW[恫r 4 23イ。ヌ? ????%O0?60% - :_欠eW[恫r 5 23届ン? ????%O4?60% - :_欠eW[恫r 6  23惕? ????%?~vRヤk+?h? 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